【摘要】 目的建立中药材独活中蛇床子素和异欧前胡素的RP HPLC含量测定方法。方法加6倍量体积的甲醇40℃水浴下提取2次,每次2 h,采用Thermo C18柱(250 mm×4.6 mm,5μm),流动相为甲醇体积分数为3%的乙酸水溶液(体积比为70∶30),流速为1.0 mL.min-1,检测波长为307nm。结果理论塔板数以蛇床子素和异欧前胡素计算均不低于24 000,两物质的色谱峰之间及与相邻峰之间分离度均大于1.5,对称因子均在0.95~1.05之间。蛇床子素和异欧前胡素的线性范围分别为29.9~180 mg.L-1和1.51~18.1 mg.L-1,平均回收率为104.3%和100.0%。蛇床子素和异欧前胡素的精密度和重复性实验结果分别为0.16%和0.85%、1.3%和2.0%。稳定性试验表明,样品溶液中2种成分在24 h内稳定性良好。结论建立的方法灵敏、准确、重现性好,可用于中药材独活的质量控制。更多还原

【Abstract】 Objective To establish a reversedphase HPLC method for determination of osthole and isoimperatorin in Angelica pubescens.Methods The sample was separated on a Thermo C₁₈ column（（250 mm）×（4.6 mm）,（5 μm））using methanol-3% acetic acid solution（V∶V=70∶30）as the mobile phase at a flow rate of(1.0 mL·min^-1).The detection was set at 307 nm.Results The calibration curve was linear in the range of 29.9-(180.0 mg·L^-1)（r=0.999 9）for osthole and 1.51-(18.1 mg·L^-1) for isoimperatorin（r=1.000）. The inner-day RSD was 0.16% and 0.85%,and the average was 104.3% and 100.0% for osthole and isoimperatorin,respectively.It was stable within 24 hours.Conclusions This method is accurate,simple and sensitive,and it can be used for the quality control of Angelica pubescens.更多还原