【摘要】 目的建立一种实时荧光定量PCR方法,并用该方法检测乳腺癌患者血浆循环DNA水平。方法用QIAamp血液DNA抽提试剂盒提取61例乳腺癌患者和25例健康妇女血浆循环DNA,用实时荧光定量PCR技术(SYBR Green I染料法)进行定量,结果用设定临界域值时的循环数的值(Ct值)表示,并对定量结果进行ROC曲线分析。结果:乳腺癌患者Ct值(27.71±1.41)显著低于健康对照(30.37±1.32,P<0.001),说明乳腺癌患者血浆循环DNA水平显著高于健康对照组;ROC曲线下面积为0.921。结论应用实时PCR技术检测乳腺癌患者血浆循环DNA水平有可能成为一种无创性乳腺癌分子诊断方法。更多还原

【Abstract】 Objective To detect the level of circulating DNA in plasma of patient with breast cancer,using a real-time florescence quantitative PCR method.Methods Plasma DNA in 61 patients with breast cancer and in the 25 controls was extracted using a QIAamp blood mini kit(Qiagen) and was measured using real-time quantitative PCR(SYBR Green I dye assay).The DNA level of species was expressed as cycle number of the threshold(Ct).The plasma DNA level in two groups was analyzed by ROC curve.Results The plasma DNA level in breast cancer patients(Ct = 27.71±1.41) was significant higher than that in the control(Ct = 30.37±1.32)(P<0.001),The area under the ROC curve for plasma DNA concentration was 0.921 for patients with breast cancer versus the controls individuals.Conclusions Quantification of plasma circulating DNA,using real-time quantitative PCR,may be a valuable noninvasive diagnostic tool in discriminate breast cancer from healthy individuals.更多还原