【摘要】 目的分析引起新生儿感染的输血传播病毒(TTV)ORF1区基因序列特征。方法应用巢式PCR技术检测外周血TTV-DNA,15例中国新生儿确诊为TTV感染;对新生儿感染的TTV(中国株,C01~C15)ORF1区进行基因序列测定。结果引起新生儿感染的中国株TTV与日本株N22比较,同源性达87.1%~97.7%,但存在着点突变,如112、113位点的GG→TT和236~240位点的TTATC→CCTAT。结论TTV中国株与日本株具有同源性,某些TTV基因突变可增强其致病性,可导致新生儿肝功能损害和结合胆红素升高。更多还原

【Abstract】 Objective To analyze the mutations in nucleotide sequences of transfusion transmitted virus(TTV) in neonatal infection.Methods Neonatal serum TTV-DNA was detected by a nested PCR technique.Fifteen Chinese neonates with positive TTV-DNA were diagnosed as TTV infection.ORF1 sequences of TTV-DNA from these neonates were determined.Results Homology of Chinese TTV(C01-C15) and Japanese TTV(N22)isolated ranged from 87.1%-97.7% at nucleotide level,but there were point mutations in Chinese TTV,such as GG→TT in locus 112 and 113,TTATC→CCTAT in locus 236-240.Conclusions Chinese and Japanese TTV isolated had the same genotype.Some gene mutations may increase the TTV pathogen,and result in neonatal hepatitis syndrome or hyperbilirubinemia.更多还原