【摘要】 目的探讨难治性肺炎支原体肺炎(MPP)患儿T细胞活化功能状态。方法采用免疫荧光标记和流式细胞仪技术对26例难治性MPP患儿外周血T细胞亚群的表面标记和活化后表面分子的表达进行分析,并以18例健康儿童外周血作为对照。结果与对照组比较,难治性MPP患儿CD4+T细胞、CD4+/CD8+值、CD3+/HLA-DR+活化T细胞和T细胞活化后CD3+/CD25+细胞的表达率均显著降低(P均<0.01);难治性MPP患儿CD8+T细胞的表达率明显高于对照组(P<0.01);而CD3+T细胞的表达率在两组间无显著差异(P>0.05)。结论难治性MPP患儿存在T细胞免疫功能紊乱和T细胞活化功能障碍。更多还原

【Abstract】 Objective To study disfunction of activated T cell from peripheral blood and explore its role on pathogenesis in patients with refractory mycoplasma pneumoniae pneumonia （MPP）.Methods T cell subsets and expression of membrane molecule on activated T cell in peripheral blood were measured in 26 refractory MPP patients by using immunofluorescence and flowcytometry,and compared with those of 18 control healthy children.Results The percentage of CD4⁺ cell from peripheral blood in refractory MPP children was significantly lower than that in control group（P<0.01）,the expressions of CD3⁺/HLA-DR⁺ and CD3⁺/CD₂5⁺ on T cell were also lo-wer（all P<0.01）.The percentage of CD8⁺cell was significantly higher in refractory MPP children than that in control group（P<0.01）,and a ratio of CD4⁺/CD8⁺ was disproportion（P<0.01）,but there was no significant difference in CD3⁺ cell between the patients and control group（P>0.05）.Conclusions There are disfunctions of T cell in refractory MPP patients in acute period,and the abnormal T cell activated disfunction may play an important role on pathogenesis.更多还原