【摘要】 目的研究血小板活化因子乙酰水解酶(PAF-AH)基因多态性与支气管哮喘儿童发病的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术,对53例哮喘及35例正常对照组儿童PAF-AH第11外显子Ala379Val位点基因多态性进行分析。结果哮喘组患儿基因组型频率和等位基因频率与对照组比较均无显著性差异(P均>0.05)。结论PAF-AH第11外显子Ala379Val基因突变与儿童支气管哮喘发病无关。更多还原

【Abstract】 Objective To study the correlative association between platelet-activating factor acetylhydrolase(PAF-AH) genetic polymorphism and bronchial asthma in children.Method Genetic polymorphism was analyzed in 53 asthmatic children and 35 healthy children with polymerase chain reaction-restriction fragment length polymorphism(PCRRFLP) technique.Result There were no significant differences between asthmatic group and normal control group about distribution frequency of genotypes and alleles(all P>0.05).Conclusion PAF-AH-Ala379Val gene mutation is unrelated to bronchial asthma in children.更多还原