【摘要】 目的回顾分析26例白血病和实体瘤患儿外周血干细胞采集效果及其对受体造血恢复影响,筛选可能与采集效率提高和受体造血恢复相关的因素。方法总结26例供体的49次粒系集落刺激因子(G-CSF)动员后的外周血采集过程,统计分析供体动员剂量、采集时外周血白细胞、收获的单个核细胞(MNC)和CD34+细胞数、受体造血恢复三者间的相关性。结果26例供体均能很好耐受动员和采集过程。采集物MNC和CD34+细胞中位数分别为4.5×108/kg和1.9×106/kg。动员剂量与采集物中MNC数呈正相关,与采集物CD34+细胞数无相关。采集当天供体外周血白细胞总数对采集细胞量无影响,而MNC水平与CD34+细胞采量正相关。结论受体的造血恢复与采集物中的CD34+细胞数可能存在相关性。因此这类患儿的采集时有必要动态监测其动员时的外周血CD34+细胞水平,确定最佳采集时间,以获取足够的CD34+细胞采量。更多还原

【Abstract】 Objective Data on the leukapheresis from 26 pediatric patients with hematologic or solid malignancies was retrospectively evaluated to screen predictive factors affecting the efficacy of peripheral blood stem cell（PBSC） collection from donors,as well as hematopoietic recovery in recipients.Methods We present our experience with 49 apheresis from 26 granulocyte-colory Stimulating factor mobilized donors and analyzed the correlations between the mobilization,the leukocyte count in the donor peripheral blood and the MNC and CD₃₄⁺ cell yields in collecting products and the neutrophil and platelet recovery of recipients.Results The process of mobilization and apheresis were well tolerated by our pediatric donors.The median numbers for harvested MNCs and CD₃₄⁺ cells were 4.5×10⁸/kg and 1.9×10⁶/kg of recipient body weight,respectively.Mobilizing dose positively affected the number of mononuclear ceus（MNC） but not CD₃₄⁺ cells in the apheresis products.The CD₃₄⁺ cell number in the apheresis product was influenced significantly by donor circulating MNC on the day of harvest and correlated with recipient′s engraftment after PBSC was reinfused.Conclusions The MNC yield was stable and met with the demand for autologous stem cell transplantation while the CD₃₄⁺ cell number varies obviously from each donor.Since a rapid engraftment was associated with a high number of CD₃₄⁺ cells collected,which was in turn predicted by the level of the pre-apheresis CD₃₄⁺ cells in the peripheral blood of donors,it is necessary to monitor the donors′ CD₃₄⁺ cell during mobilization to determine the optimal time for apheresis.J Appl Clin Pediatr,2006,21（3）:148-150更多还原