【摘要】 采用3株北虫草(Cordceps militaris)组织分离株及2株单孢分离株在PDA培养基上进行15次继代培养。结果表明:组织分离株在继代培养中可发生明显角变。将角变区菌丝分离培养,根据菌落形态,可将菌落分为5种类型。Ⅰ、Ⅱ型菌落具有角变频率高、角变面积大的特点,多出现在组织分离株继代的最初几代。无角变的Ⅲ、Ⅳ、Ⅴ型分布在单孢株和组织分离株的继代的后几代。出发菌株决定角变类型及分布。Ⅰ型比Ⅱ型子实体产量高。菌株具有高角变频率时期子实体形成能力强。更多还原

【Abstract】 Three isolates of Cordceps militaris from tissue isolations and two single-spore isolates from them were subcultured on the PDA medium for fifteen times.The results showed that substantial sectors were detected by morphology of sectorial colonies.Hyphae in sectorial area were isolated and cultured.Five colonial types were classed by colonial morphology.Type Ⅰand Ⅱ colonies had the characters of higher sectoreial frequencies and bigger sectorial variation area,which were usually found at earli-er generations of tissue isolates.But the other types without sectors were found in single-spore isolates and later generations of tissue isolates.Starting strains decided colonial types and their distribution.Type Ⅰcolonies had higher fruit-body yield than type Ⅱ.Isolations had higher abilities of fruit-body when they had higher sectorial frequencies.更多还原