【摘要】 目的探讨非小细胞肺癌(non-small lung cancer,NSCLC)中survivin和bcl-xl基因蛋白的表达及其相关性。方法选择2004年2月至2005年10月聊城市人民医院收治的32例NSCLC患者,应用组织芯片技术制成32例NSCLC组织芯片,采用免疫组织化学技术检测同期30名正常成人肺组织及32例NSCLC患者肺组织中survivin及bcl-xl基因蛋白的表达。结果survivin和bcl-xl基因蛋白在NSCLC组织中表达阳性率分别为71·9%和81·3%,显著高于正常肺组织(P<0·05)。survivin蛋白表达与肺癌TNM分期、淋巴结转移及细胞分化程度密切相关,但与肿瘤组织学类型、年龄、性别无关(P>0·05)。bcl-xl基因蛋白表达与细胞分化程度密切相关(P<0·05),与肿瘤TNM分期、淋巴结转移、肿瘤组织学类型、年龄、性别无关(P>0·05)。survivin与bcl-xl基因蛋白过表达呈正相关(P<0·05)。结论survivin和bcl-xl基因蛋白在NSCLC中表达增强且呈正相关,可能与NSCLC发生有关,survivin在NSCLC淋巴结转移方面可能起着重要作用,提示NSCLC预后不良。更多还原

【Abstract】 Objective To investigate the expression of survivin and bcl-xl in non-small cell lung cancer(NSCLC)and their relationship.Methods The NSCLC tissuechips including 32 cores were constructed and expression of survivin and bcl-xl was examined by immunohistochemical technique in 30 normal bronchial epithelium and 32 primary NSCLC.Results The positive rate of survivin and bcl-xl protein in NSCLC was 71.9% and 81.3% respectively,significantly higher than that in the normal ones(P<0.05).The expression of survivin protein was closely related to TNM stages(P<0.05),lymph node involvement(P<0.05)and cell differentiation(P<0.01),but not to histological classification,gender and ages(P>0.05).The expression of bcl-xl protein was closely related to cell differentiation(P<0.05),but not to TNM stages,lymph node involvement,histological classification,gender and ages(P>0.05).The over-expression of survivin and bcl-xl was positively correlated(P<0.05).Conclusion The up-regulation and positive correlation of survivin and bcl-xl are probably related to the tumor genesis of NSCLC.The over-expression of survivin may play an important role in the lymph node metastasis and indicates the poor prognosis of NSCLC.更多还原