【摘要】 目的通过流式细胞仪(FCM)技术测定胸水脱落细胞细胞核的脱氧核糖核酸(DNA)含量,并将此方法与传统的病理学方法,肿瘤标记物癌胚抗原(CEA)的检测相比较,以鉴别胸水的良恶、性。方法病例选自2003-03~2004-03苏州大学附属第二医院呼吸内科的住院患者,留取新鲜的胸腔积液标本,以肝素抗凝,荧光染料染色后,用流式细胞仪检测细胞核DNA倍体,分析细胞的增殖能力。结果根据临床检查将胸水分为良性和恶性两组,在恶性胸腔积液组中,87·5%的标本可以检测到DNA异倍体,在良性胸腔积液中,90%的标本表现为两倍体。结论流式细胞仪DNA倍体测定使得病理诊断有了可依据的客观指标,是病理学检查的有益补充,与CEA联合测定可以提高恶性胸腔积液的检出率。更多还原

【Abstract】 Objective To discuss the diagnosis value of the DNA heteroploid detection with flow cytometry(FCM)between themalignant and benign pleural effusion.At the same time detected CEA.Methods During March 2003 to March 2004,there were 47 inpatients with pleural effusion,collected the fresh pleural effusion and put it in the bottle with heparin,after stained the cell,used flow cytometry to detect the nuclei heteroploid,From the histogram,could obtain some indexes such as DI 、SPF、PI.Results In malignant group,the sensitivity of FCM DNA is 87.5%;in the benign group,the specificity of FCM DNA is 90%.Conclusion The DNA heteroploid detection with flow cytometery has good clinical application to diagnosis of the malignant pleural effusion. And its sensitivety,specificity were satisfactory. If associated with CEA detection the clinical application was better.更多还原