【摘要】 目的观察沙利度胺和三氧化二砷(As2O3)对急性非淋巴细胞白血病(ANLL)细胞分泌血管内皮生长因子(VEGF)的影响,并观察二者是否有协同性。方法对2001-10~2003-02中国医科大学第二临床学院的23例ANLL病人的骨髓,用Ficoll淋巴细胞分离液分离单个核细胞(MNC),培养于RPMI1640培养液中,于37℃、5%CO2饱和湿度条件下传代培养。以2×109/L的细胞加入24孔培养板。分为空白对照组、沙利度胺组(300μg·mL-1)、As2O3组(25μmol/L)和联合用药组,连续培养72h。用ELISA法检测上清液VEGF质量浓度。结果4组VEGF质量浓度分别为(1582·67±281·51)ng/L、(1206·29±264·87)ng/L、(941·72±236·34)ng/L和(639·12±211·98)ng/L。用药组与空白对照组及联合用药组与单一用药组之间差异均有显著性(P<0·05)。结论(1)白血病细胞能自分泌VEGF。(2)沙利度胺和As2O3均能抑制ANLL细胞分泌VEGF。(3)两者联合应用起协同作用。更多还原

【Abstract】 Objective To investigate the effect of thalidomide and As₂O₃ to the secretion of VEGF of the cell of ANLL and study if there is coorperation in thalidomide and As₂O₃.Methods From October 2001 to February 2003,23 patients with ANLL were analyzed.Heparinized marrow was taken,then it was putt into Ficoll-isopaque solution to be purified for MNC,which was cultured in the RPMI 1 640 medium,placed in 5% CO₂ saturation and 37℃ to proliferation.Choosing the cells in logarithm growth phase,adding cultural solution to abstaining the cellular density of 2×10⁹/L and then putting it into 24-well plates.There are 4 groups :contrast group,thalidomide（300 μg·mL -1 ）group,As₂O₃（25 μmol/L）group,and thalidomide（300 μg·mL -1 ）andAs₂O₃（25 μmol/L）group,cultured for 72 hours.Examine the concentration of VEGF in upper liquid with ELISA method.Results The concentration of VEGF in upper liquid of 4 groups were respectively（1 582.67±281.51）ng/L、（1 206.29±264.87）ng/L、（941.72±236.34）ng/L、（639.12±211.98）ng/L.There were statisticd difference（P<0.05）.Conclusion （1）Leukemia cell can secrete VEGF.（2）Thalidomide and As₂O₃ can both lower the expression of VEGF in leukemic cells.（3）Thalidomide can enhance the function of anti-leukemia of As₂O₃.更多还原