【摘要】 目的通过对健康老年人与年轻人外周血CD3+、CD4+、CD8+T细胞及CD4+、CD8+T细胞CD28表达水平的比较性研究,探讨免疫衰老的细胞学机制。方法采用三色免疫荧光标记流式细胞术分析年轻组(21~59岁)与老年组(≥60岁)外周血T淋巴细胞亚群水平。结果老年组CD3+、CD8+CD28+、CD8+T细胞明显低于年轻组(P<0·05~0·01),CD4+CD28-、CD8+CD28-T细胞明显高于年轻组(P<0·01)。结论老年人CD8+T细胞CD28+、CD28-的表达水平及CD4+T细胞CD28-的表达水平均随着增龄而有明显改变,CD28+表达水平下降,CD28-表达水平增高,预示着老年人免疫系统功能的降低,提示CD4+CD28-、CD8+CD28+、CD8+CD28-T细胞可能是评价免疫衰老的潜在生物标志。更多还原

【Abstract】 Objective To explore the change of the cellular immune function with aging through detecting the levels of CD3~ + ,CD4~ + ,CD8~ + T-lymphocytes and the expression of CD28 on CD4~ + 、CD8~ + T cells in the peripheral blood. Methods Three-color fluorescent flow cytometry was used to analyze the percentage of peripheral blood T cell subsets in the young and elderly groups. Results CD3~ + ,CD8~ + CD28~ + ,CD8~ + T cells in the peripheral blood of the elderly group were significantly lower than those in the young group(P<0.01 or P<0.05), CD4~ + CD28~ - ,CD8~ + CD28~ - T cells in the peripheral blood of the elderly group were significantly higher than those in the young group(P<0.01). Conclusions The expression of CD28~ + ,CD28~ - of the CD8~ + T cells and CD28~ - of the CD4~ + T cells in the peripheral blood are changed significantly with aging. The decreased CD28~ + expression and the increased CD28~ - expression of T cells play an important role in the immunosenescence, suggesting that CD4~ + CD28~ - ,CD8~ + CD28~ + , CD8~ + CD28~ - T cells may be potential biomarkers for evaluating immunosenescence.更多还原