【摘要】 目的:研究dpc4基因在金黄地鼠颊囊癌变过程中的表达特征,探讨其与口腔鳞癌的发生、发展之间的关系及临床意义。方法:用免疫组化技术检测23例金黄地鼠颊囊正常上皮、21例单纯增生上皮、27例异常增生上皮、25例鳞癌组织中DPC-4的表达。结果:DPC-4在正常黏膜上皮组织的细胞质内呈稳定性、强阳性表达,在细胞质内出现棕褐色颗粒;在单纯增生上皮中表达与正常黏膜组织相似;在上皮异常增生组织表达降低,阳性率为74.1%;在鳞癌中的表达亦明显降低,阳性率为48%,且与正常黏膜、异常增生黏膜相比有显著性差异(P<0.01)。结论:Smad4的缺失促进了口腔黏膜鳞癌发生发展。更多还原

【Abstract】 Objective:To elucidate the role of dpc4 gene in the development of squamous cell carcinoma. Methods:DMBA at 5 g/L was locally applied in check pouch 3 times a week for 6,9 and 12 weeks respectively in three groups of golden hamasters to induce carcinoma.The animals were sacrificed after DMBA application.Normal controls were the check pouch samples of golden hamasters without any treatment and negative controls were established by local application of acetone for 12 weeks.DPC4 4 expression was examined by immunohistochemical staining.Results:DPC4 expression was observed in all(22) cases of the normal epithelium samples(100%),20 of the 21 cases of simple hyperplasia(95.2%),20 of the 27 cases of abnormal hyperplasia(74.1%) and 12 of the 25 cases of squomous cell carcinoma(48%).Conclusion:dpc4 may play an important role in the development of oral squomous cell carcinoma.更多还原