【摘要】 目的探讨基质金属蛋白酶和Ⅳ型胶原与成釉细胞瘤复发、侵袭的关系。方法采用免疫组织化学LABC方法检测28例成釉细胞瘤组织中MMP-2、TIMP-2和Ⅳ型胶原的表达,并用RT-PCR方法检测MMP-2、TIMP-2和MT1-MMP在20例成釉细胞瘤的表达。结果MMP-2、TIMP-2和Ⅳ型胶原蛋白在成釉细胞瘤的阳性表达率分别为92.9%、75.0%和42.9%,Ⅳ型胶原阳性着色部位均位于基底膜及部分肿瘤细胞的胞质,MMP-2和TIMP-2阳性着色部位均位于细胞质,分布于瘤组织各层;复发性成釉细胞瘤Ⅳ型胶原的阳性表达率明显低于原发性成釉细胞瘤(P<0.01)。MMP-2、MT1-MMP、TIMP-2的mRNA在成釉细胞瘤组织中均有表达,复发性成釉细胞瘤的TIMP-2、MT1-MMP相对含量均显著高于原发性成釉细胞瘤(P<0.01)。结论基质金属蛋白酶MT1-MMP表达增加和Ⅳ型胶原在基底膜表达缺失与成釉细胞瘤的复发密切相关,MMP-2活性增加和基底膜Ⅳ型胶原降解增多可能是成釉细胞瘤局部侵袭的机制之一。更多还原

【Abstract】 Objective:To investigate the relationship between matrix metalloproteinase and collagen Ⅳ expression and ameloblastoma invasion and recurrence.Methods:Immunohistochemical LABC method was used in detecting the expression of matrix metalloproteinase-2(MMP-2), tissue inhibitor of metalloproteinase-2(TIMP-2) and collagen Ⅳ in 28 cases of ameloblastoma. Expression of MMP-2, TIMP-2 and membrane type 1-matrix metalloproteinase(MT1-MMP)in 20 cases of ameloblastoma was examined by RT-PCR.Results:The total positive expression rate of MMP-2, TIMP-2 and collagen Ⅳ in ameloblastoma was 92.9%, 75.0% and 42.9% respectively. The positive expression of collagen Ⅳ was mainly located in the basement membrane and the cytoplasm of some cells. The staining of MMP-2 and TIMP-2 was mainly located in the cytoplasm of all layers of the tumor. The positive expression rate of collagen Ⅳ in basement membrane of recurrent ameloblastoma was significantly lower than that of the primary(P<0.01). All investigated cases expressed mRNA of MMP-2, MT1-MMP and TIMP-2. The mRNA levels of TIMP-2 and MT1-MMP in recurrent ameloblastoma were significantly higher than those in primary ameloblastoma respectively(P<0.01).Conclusion:Increased expression of MT1-MMP and absent expression of collagen Ⅳ in basement membrane may be associated with the recurrent potentiality of ameloblastoma, and the augment in MMP-2 activity and degradation of collagen Ⅳ may be a reason of ameloblastoma invasion.更多还原