【摘要】 目的:在分子水平探讨芦荟凝胶抑制大鼠Ⅱ度放射性皮炎发生的机理。方法:首先建立SD大鼠裸露背部10%Ⅱ度放射性皮炎模型,随机分成两组,对照组为自然愈合组,实验组为芦荟外涂组,实验组每日进行鲜芦荟凝胶对辐射伤口区的外涂,每日两次。辐射伤后2、6、10、18、24、34d各活杀大鼠10只,每组各5只。采用免疫组织化学方法并利用图像分析技术,分别记录伤口局部ICAM-1、EGF和bFGF的表达量。结果:实验组ICAM-1的表达总体均数低于对照组的表达P<0.01,差异有显著意义。实验组EGF和bFGF的表达总体均数高于对照组的表达,P<0.05,差异有显著意义。结论:芦荟凝胶对大鼠Ⅱ度放射性皮炎的发生有抑制作用,机理与ICAM-1分泌的抑制及EGF,bFGF分泌的促进相关。更多还原

【Abstract】 Objective:To study the mechanism of aloe gel on depression of radioactive dermatitis vesicular reaction in rats at molecular level.Methods: The rats models of 10% II radioactive dermatitis vesicular reaction on nude back were established.The rats were divided into 2 groups randomly: the normal healing group was the control group,and experimental group treated with aloe gel twice a day.At the 2^th,6^th,10^th,18^th,24^th and 34^th day after radiation,5 rats in each group were killed,the protein of ICM-1,EGF and bFGF were stained by immunohistochemistry and image analysis.Results: The total expressions of ICM-1 in experimental groups was significantly lower than that in control groups（P<0.01）.The total expression of EGF and bFGF in experimental group were significantly higher than that of control group（P<0.05）.Conclusion: The aloe gel could depress radioactive dermatitis vesicular reaction by increasing the EGF,bFGF release and decrease ICAM-1 secretion.更多还原