【摘要】 目的:探讨支气管肺癌中端粒酶活性和p53蛋白表达的意义及其与预后的关系。方法:采用PCR检测端粒酶活性,免疫组化SABC检测p53蛋白表达。并观察其5年的生存期。结果:肺癌组织中端粒酶活性与p53蛋白表达分别为80.9%和59.6%,而正常组织无表达。端粒酶活性、p53蛋白表达均与肺癌临床分期、病理类型、淋巴结转移无显著性相关。肺癌p53蛋白表达与无p53蛋白表达的5年生存率比较无显著性差异,端粒酶活性表达与无端粒酶活性表达的5年生存率比较有显著性差异,有端粒酶活性表达的患者,其生存率较低。结论:端粒酶活性和p53蛋白表达与肺癌发生有关,两者均可作为肺癌早期肿瘤标志物;p53蛋白与肺癌临床预后无明显相关,有端粒酶活性表达肺癌患者预后较差。更多还原

【Abstract】 Objective:To investigate the expression of human telomerase and p53 in the primary lung cancer tissues and normal adjacent tissues their significance in clinical prognosis.Methods: Telomerase repeat amplication protocol was used to detect telomerase activity,immunohistochemical method was used to detect the expression of p53 in the lung cancer tissues and normal adjacent tissues of 47 patients,and then investigate survival rate for five years.Results: The positive rates of telomerase and p53 in lung cancer tissues were 80.9%,59.6% respectively.Those of normal adjacent tissues were 0.0%.The expression of telomerase and p53 was not significantly related to histological type,clinical stage,lymph node metastasis.The expression of telomerase is not signicantly related to expression of p53.Five year survival rate is not related to the expression of p53,but is related to the expression of telomerase.Conclusion: Telomerase and p53 play an important role in development of lung cancer.Both are early diognosis markers of lung cancer.p53 is not related to clinical prognosis.The prognosis of patients with lung cancer,in which the expression of telomerase is positive,is poor.更多还原