【摘要】 目的建立经口感染弓形虫RH株速殖子垂直传播小鼠模型。方法将54只妊娠0天的BALB/c孕鼠随机分为6组,于妊娠第8天,分别用0,1000,2000,4000,8000,16000个弓形虫速殖子灌胃感染,妊娠第18天处死,记录活胎率,测定母鼠肝、胎盘和胎仔脑组织弓形虫抗原。结果经ELISA检测弓形虫循环抗原证实,孕鼠死亡率与胎盘和胚胎感染率均随感染剂量的增加而升高,而活胎率下降。1000,2000组的胎盘、胚胎未发生感染,4000组的胚胎感染率5.13%,16000组的胚胎感染率20.00%,但孕鼠的健康状况差、死亡率高,均不适宜建立弓形虫垂直传播动物模型。综合分析表明,8000组孕鼠既有较高的存活率,又可保证胎盘和胚胎较高的感染率。结论每只孕鼠灌胃感染8000个速殖子为建立弓形虫垂直传播小鼠模型的适宜剂量。更多还原

【Abstract】 Objective To establish the mouse model of vertical transmission by orally infected tachyzoites of Toxoplasma gondii RH strain. Methods Fifty-four pregnant mice were randomly divided into 6 groups (n=9 in each group), at the day 8 of gestation, the mice were orally infected with 0, 1 000, 2 000, 4 000, 8 000, 16 000 tachyzoites of T.gondii RH strain, respectively. At the day 8 of gestation, the mice were killed and their uteri were anatomized for observing survival rate of embryos and the Toxoplasma antigene in pregnant mice livers, placentas and fetuses were detected by ELISA. Results The mortality of the pregnant mice and the infectious rate of the placentas and embryos were a dose-dependent rise.The mice of 1 000 and 2 000 groups were not infected;the infectious rate of embryos in 4 000 group was lower (5.13%) and that of 16 000 group was the highest (20.00%).In a word,the survival rate and infectious rate of placentas and embryos in 8 000 group were higher than that in the other groups. Conclusion Tachyzoites of 8 000 oral infection per mouse is a suitable dose which can be applied to establish the mouse model of vertical transmission.更多还原