【摘要】 目的建立球形幽门螺杆菌(Hp)感染BALB/c小鼠的疾病模型并进行初步研究。方法禁食12 h后,分别于第0,3,6,9 d共4次以抗生素诱变后的球形Hp对SPF级BALB/c小鼠给予灌胃处理。于末次灌胃4周后禁食36 h取胃组织作细菌学(组织尿素酶试验、细菌培养)和病理学检测。结果球形Hp灌胃4周后,实验组BALB/c小鼠组织尿素酶试验阳性率达80%、Hp培养阳性率达100%,病理学检测结果显示70%实验组小鼠胃黏膜出现糜烂,与生理盐水对照组比较均有显著意义(P<0.05)。结论成功建立了球形Hp-BALB/c小鼠感染的疾病模型,证实了其在实验动物体内的可致病性。更多还原

【Abstract】 Objective To establish stable disease model infected by coccoid forms of Helicobacter pylori in BALB/c mice and progress the initial study.Methods After 12 h fasting,the experimental group was given coccoid forms of Hp(mutagenized by antibiotic) via gavage 4 times(0.5×10~9/0.5 ml each mouse).On week 4 after the last gavage,mice were sacrificed.Histological change and colonization of Hp in the gastric mucosa were detected by urease test and culture of Hp.Results To the experimental group,the positive rate of urease test of gastric mucosa was 80%(8/10),the positive rate of culture of Hp was 100%(10/10),and in 70%(7/10)mice,different degrees of erosion were observed.They were all higher than those of control group(P<0.05).Conclusion Coccoid forms of Hp infected disease model can be successfully made in BALB/c mice,and its pathogenicity is confirmed in vivo.更多还原