【摘要】 目的探讨焦炉作业工人苯并(a)芘暴露与外周血淋巴细胞DNA损伤的关系及其特征。方法选取343名焦炉工人和40名对照,应用彗星试验检测外周血淋巴细胞DNA损伤。分别采用定点采样和个体采样方法,使用高效液相色谱法测定环境苯并(a)芘浓度。利用调查表收集研究对象年龄、工龄、从事现工种时间、吸烟、饮酒、职业史等信息。结果定点采样,苯并(a)芘浓度呈现炉顶>炉侧>炉底>对照的趋势,分别为(1.26±0.65),(0.11±0.38),(0.02±0.003),0.01μg/m3。个体采样苯并(a)芘浓度呈现炉侧拦焦侧>炉顶>炉侧推焦侧>炉底>辅助工区>对照的趋势,分别为(1.43±0.06),(0.35±0.02),0.10,(0.05±0.007),(0.04±0.01),0.02μg/m3。焦炉工人外周血淋巴细胞慧星尾长(几何均数)分别为(16.94±2.59),(17.52±2.39),(10.78±2.48),(6.89±1.90),olive尾矩(几何均数)分别为(4.60±1.29),(4.57±1.17),(2.79±1.11),(1.61±0.56),炉顶工、炉侧工、炉底及辅助工均显著高于对照组,差异有显著性(P<0.05),炉顶工与炉侧工之间差异不显著(P>0.05),但均显著高于炉底及辅助工,差异有显著差异(P<0.05)。炉顶工、炉侧工、炉底及辅助工与对照相比,外周血淋巴细胞慧星尾长和olive尾矩阳性的危险度OR值分别为29.66,28.90,9.97和27.24,26.04,9.32,有随个体外暴露苯并(a)芘浓度增高而增高的趋势。结论焦炉工外周血淋巴细胞DNA遗传损伤与个体苯并(a)芘暴露趋势一致。环境个体采样及彗星试验可作为焦炉工人PAHS暴露和细胞遗传损伤的监测指标。更多还原

【Abstract】 Objective To investigate Benzo(a)pyrene(Bap) concentration in the ambient air and DNA damage of peripheral blood lymphocytes in coke oven workers exposed to polycyclic aromatic hydrocarbons(PAHs).Methods A total of 343 coke oven workers and 40 controls without occupational PAHs exposure were investigated.Bap concentration in the ambient air,which was collected respectively by fixed point and individual ways,was assayed by high performance liquid chromatography(HPLC).Comet assay was used to evaluate the DNA damage levels in peripheral blood lymphocytes.Personal information including occupational history,age,sex,smoking and drinking status was collected by questionnaire.Results Bap concentration(μg/m~3) in the ambient air collected by fixed point way in oven-top,-side,-bottom and control area was(1.26±0.65),(0.11±0.38),(0.02±0.003) and 0.01 respectively.Bap concentration(μg/m~3) in the ambient air collected by individual way in oven-top,-side,-coke side,-bottom,-utility operator and control area was(0.35±0.02),0.10,(1.43±0.06),(0.05±0.007),(0.04±0.01) and 0.02 respectively.The comet tail length and olive moment of lymphocyte of coke oven workers in oven-top,-side,-bottom and utility operators [(tail length:(16.94±2.59),(17.52±2.39),(10.78±2.48).olive moment:(4.60±1.29),(4.57±1.17),(2.79±1.11)] were significantly higher than those in controls(tail length:(6.89±1.90)].olive moment:1.61±0.56)(P<0.05),and in order of oven-top and side workers>oven-bottom workers and utility operators>controls(P<0.05).The coke oven workers had significantly higher risk in DNA damage in oven-top,-side,-bottom and utility operators(comet tail length and olive moment plus adjusted OR was 29.66,28.90,9.97 and 27.24,26.04,9.32 respectively).There was dose-response relationship between external exposure Bap concentration and DNA damage.Conclusion There is same order between DNA damage in peripheral blood lymphocytes and individual Bap exposure of coke oven workers.Bap individually exposed concentration and comet assay can show the levels of PAHs exposure and PAHs-induced genotoxicity in coke oven workers.更多还原