【摘要】 目的探讨人乳头瘤病毒16型(HPV16)E2/E6癌基因在宫颈癌发生中的作用。方法采用以医院为基础的1∶1配比病例对照研究,应用热启动PCR法检测经病理诊断确诊的74例宫颈鳞癌新发病例,及同期就诊的74例宫颈良性肿瘤患者的高危型HPV16 E2/E6癌基因的状况。结果病例组HPV16 E2、E6阳性率(41.89%,52.70%)高于对照组(17.57%,21.62%),差别有统计学意义,OR分别为3.250(1.471-7.178)和3.875(1.824-8.233);病例组HPV16 E2缺失率较对照组略高。结论宫颈癌的发生与HPV感染,特别是高危型HPV16的感染密切相关;HPV16 E2基因缺失、E6基因高阳性率在宫颈癌的发生发展中起重要作用。更多还原

【Abstract】 Objective To explore the role of human papillomavirus type 16(HPV16) E2/E6 oncogene in cervical cancer.Methods This study was a hospital-based case-control study in Tumour Hospital of Shanxi province during 2001 to 2002.Seventy-four women with invasive cancer and controls with hysteromyoma were matched to cases in a 1∶1 ratio.The presence of HPV16 E2/E6 oncogenes was tested in cervical tissue using PCR. Results The expressions of HPV16 E2/E6 in patients(41.89%,52.70%)were significantly higher than that in controls(17.57%,21.62%),OR were 3.250(1.471-7.178) and 3.875(1.824-8.233);absence of HPV16 E2 in patients was slightly higher than in controls.Conclusion The infection of HPVs,especially high-risk HPV16 is related to the development of cervical cancer;the absence of HPV16 E2 and the expression of HPV16 E6 is more significant to cervical cancer.更多还原