【摘要】 目的建立雷公藤甲素脂质体中雷公藤甲素的含量测定方法。方法色谱柱μ-Bondapak C18(250mm×4.6mm,5μm);检测波长:219nm;流动相:甲醇-水-异丙醇(4:3:1);流速:1.0mL/min:柱温:30℃。结果雷公藤甲素与制剂中的辅料达到良好分离,雷公藤甲素在0.8—3.2μg范围内与峰面积有良好的线性关系(r=0.9998);平均回收率为97.65%,RSD为0.42%。结论此方法灵敏、准确、重现性好。更多还原

【Abstract】 Objective To establish a method to determine the content of thyroxin in liposome of Tripterygium Wilfordii.Methods Chromatographic column ?-Bondapak C 18(250mm*4.6mm,5?m);wavelength-testing:219nm;mobilephase:methylalcohol-water-isopropanol(4:3:1);flow rate:1.0ml/min;column temperature:30℃.Results Thyroxin of tripterygium wilfordii and auxiliary ingredients in the pharmaceutics separate satisfactorily.Thyroxin of tripterygium wilfordii has satisfactory linear relation with peak area between 0.8-3.2?g(r=0.9998);The average rate of recovery is 97.65%,RSD=0.42%.Conclusion The method is sensitive,accurate with good reproduction.更多还原