【摘要】 目的制备抗β-溶血性链球菌、G簇溶血性链球菌、金黄色葡萄球菌3种咽喉炎常见菌IgY抗体,并分析其特性。方法采用超声波破碎法将3种咽喉菌制备成可溶性抗原液,免疫产蛋母鸡,从鸡蛋黄中提取IgY抗体,应用免疫电泳、ELISA、SDS-PAGE检测抗3种咽喉菌IgY抗体的特性及热稳定性。结果抗原免疫母鸡10d后,鸡蛋黄中即有抗3种咽喉菌的IgY抗体产生,60d时抗体滴度高达1∶102400,IgY抗体滴度在室温可保持5~6个月不变。结论制备的抗3种咽喉菌IgY抗体滴度高,特异性强,稳定性好。更多还原

【Abstract】 Objective To prepare the antibodies against three kinds of common bacteria causing sore throat,i.e.β-streptococcus hemolyticus,streptococcus hemolyticus group G and staphylococcus aureus,and anlayze their properties.Methods Prepare the three kinds of bacteria into soluble antigens and immunize to hens.Extract IgY antibodies from the yolk,analyze their property by immunoeletrophoresis,ELISA and SDS-PAGE and study their thermostability.Results The IgY against three kinds of bacteria were isolated from the yolk only 10 d after immunization.Sixty days after immunization,the IgY titer reached 1∶102 400 and showed no significant change 5-6 months after storage at room temperature.Conclusion The prepared IgY antibodies showed high titers,specificities and stabilities.更多还原