【摘要】 目的:探讨枸杞组织培养及其植株再生条件的优化。方法:应用MS培养基为基本培养基,以各种不同激素配比进行枸杞愈伤诱导、分化诱导及根的诱导。结果:以枸杞叶片为外植体,利用2,4-D(2,4-二氯苯氧基乙酸)与KT(细胞分裂素)不同配比诱导出了愈伤组织。利用6-BA(6-苄基腺嘌呤)与NAA(α-萘乙酸)不同浓度的配比组合,成功地进行了杞再生芽诱导及根系诱导。结论:以MS培养基为基本培养基,并采用各种激素的不同配比,可以优化枸杞植株的再生条件。更多还原

【Abstract】 Objective:To approach on the regeneration and propagation of Lycium barbarum L.(L.barbarum).Methods:Depending on the MS base medium mixed with different plant hormones,the calli,buds and roots of L.barbarum were induced.Results:The Chinese traditional herb of the L.barbarum calli were induced from L.barbarum leaves on the MS medium with 2,4-D(2,4-dicholrophenoxyacetic acid)(3.0 ppg)and KT(kinetin)(0.2 ppg).Depending on the MS base medium mixed with 6-BA(6-benzyl adenine)and NAA(naphthylacetic acid),the regenerated buds were successfully obtained,and the roots come out.Conclusion:The MS base medium mixed with different plant hormones could be used for the regeneration of L.barbarum.更多还原