【摘要】 本文采用靛蓝胭脂红褪色反应检测抗体是否能催化经佛波酯（PMA）激活的THP-1单核细胞产生臭氧（O₃）,并借助荧光分光光度法检测细胞内胆固醇的积聚,以及高效液相色谱法（HPLC）分析细胞内胆固醇的臭氧氧化产物5,6-secosterol生成的情况,探讨抗体催化水的氧化作用对动脉粥样病变形成潜在的影响。结果显示,THP-1单核细胞经人IgG、PMA共同孵育后,显著产生了O₃的化学信号,对靛蓝胭脂红具有明显的褪色作用,过氧化氢酶能加强这种褪色反应,而乙烯苯甲酸具有显著的抑制作用；THP-1单核细胞经人IgG、PMA及低密度脂蛋白（LDL）共同孵育后,其细胞内积聚的总胆固醇（TC）、游离胆固醇（FC）、胆固醇酯（CE）及胆固醇酯占总胆固醇的比值均明显增多,并显著产生了胆固醇的臭氧氧化产物5,6-secosterol,乙烯苯甲酸对它们均有抑制效应。以上结果表明：激活的THP-1单核细胞在抗体的催化下具有产生臭氧的能力,可能是动脉粥样硬化形成过程中一种新的重要机制。更多还原

【Abstract】 In the present study,we measured the antibody-catalyzed O₃ formation from THP-1 monocytes activated by phorbol myristate acetate （PMA） by indigo carmine bleaching reaction test,and the accumulation of cholesterol in THP-1 monocytes by fluorescence spectrophotometric method,and analyzed the cholesterol ozonation product 5,6-secosterol by high-performance liquid chromatography （HPLC）,to explore the potential effect of antibody-catalyzed water oxidation on pathogenesis of atherosclerosis.It was showed that THP-1 monocytes incubated with human IgG and PMA evidently produced an oxidant with the chemical signature of O₃ which could bleach in- digo carmine,and be intensified or inhibited respectively by catalase and vinylbenzoic acid.In the THP-1 monocytes incubated with human IgG,PMA and LDL,the intracellular accumulated total cholesterol （TC）,free cholesterol （FC）,cholesteryl ester （CE） and the CE/TC increased evidently, and the cholesterol ozonation product 5,6-secosterol was also produced markly,all of that were inhibited by vinylbenzoic acid.These results demonstrated that the activated THP-1 monocytes possess the ability to produce 03 through antibody-catalyzed water-oxidation pathway,which could be a new mechanism concerned with atheriosclerosis.更多还原