节点文献
多菌灵在黄精根茎无菌培养中的应用
Application of Carbendazim in Sterile Culture of Polygonatum Cyrtonema Hua Roots
【摘要】 本研究中比较了几种杀菌剂对黄精根茎的灭菌效果。结果表明,采用乙醇、饱和漂白粉、HgCI2等杀菌剂对黄精根茎杀菌时,微生物的污染率很高;先用3%多菌灵溶液对黄精根茎进行内吸杀菌处理45h以上,然后再用0·2%HgCI2进行灭菌10min,则黄精根茎的污染率可得到有效控制。多菌灵的处理效果与黄精根茎的大小、类型和年龄等因素有关。
【Abstract】 The effect of some kinds of bactericides have compared in this paper.The results showed that microbe pollution rate was very high,if Polygonatum cyrtonema Hua roots were dealed with ethanol,saturated bleaching power or HgCI2.Polygonatum cyrtonema Hua roots pollution rate were controlled by 3% Carbendazim at least 45h and 0.2% HgCI2 10min.The effect of Carbendazim on Polygonatum cyrtonema Hua roots has relate to the size,type and age of Polygonatum cyrtonema Hua.
【关键词】 多菌灵;
多花黄精;
污染率;
组织培养;
【Key words】 Carbendazim Polygonatum cyrtonema Hua Pollution rate Tissue culture;
【Key words】 Carbendazim Polygonatum cyrtonema Hua Pollution rate Tissue culture;
【基金】 浙江省自然科学基金资助项目(基金编号:302312)
- 【文献出处】 生物技术通报 ,Biotechnology Bulletin , 编辑部邮箱 ,2006年S1期
- 【分类号】S567.239
- 【被引频次】18
- 【下载频次】363