【摘要】 目的:为了获得黄霉素高产菌株。方法:进行了一系列的诱变筛选。以加纳链霉菌(Streptomyces ghanaesis)SgWE-11为出发菌株,采用紫外线诱变,以其自身代谢物黄霉素作为筛选因子。结果:获得一株遗传稳定且黄霉素发酵效价比出发菌株高出170.25%的菌株。对该菌株采用亚硝酸诱变,并以链霉素作为筛选因子时得到一株效价值为875u/mL的高产菌株SgWE-25,比出发菌株SgWE-11提高了4.4倍。传代结果表明,在传代的同时结合自然分离,可以保持稳定的产抗特性。更多还原

【Abstract】 Objective:In order to get some high-yield strains,different mutant modes and screening factors were used.Method:A flavomycin producing strain SgWE-11 was treated by UV and flavomycin as screening factor.Results:A stable strain was obtained with the titer of flavomycin increased by 170.25% than parent strain.Then the mutant which grow on media containing Streptomycin was treated with HNO₂,we got a high-yeild strain SgWE-25.The productivity of strain SgWE-25 reached 875u/mL which was 4.4-times.higher than that of the original of strain SgWE-11.When it was subcultured combined with natural screening,its stability was rather satisfied.更多还原