【摘要】 目的:研究无溶剂体系中脂肪酶催化红花油水解反应的时间效应。方法:采用单因素实验法,确定不同反应时间时各因素的最佳水平。结果:反应1h时的最适条件为35℃、n(水)/n(油)比35p、H 7.0、缓冲液浓度0.05mol/L、添加0.2%CaCl2;反应24h时,最适条件为26℃、n(水)/n(油)比60p、H 7.6,缓冲液浓度及CaCl2的影响不显著。结论:水解最佳条件随反应时间变化,应根据反应时间长短确定各因素的最佳水平。更多还原

【Abstract】 Objectives:To study time effects of the enzymatic safflower oil hydrolysis in a solvent free system by a lipase from Aspergillus niger.Methods: Single factor experimentation was used to investigate the optimum condition of each factor for the hydrolysis.Results: The optimum conditions for 1hours reaction were 35℃,water/oil molar ratio 35,pH 7.0,the buffer solution concentration 0.05mol/L and 0.2% CaCl₂ was used;while the optimum condition for 24 hours were 26℃,water/oil molar ratio 60,pH 7.6,the buffer solution concentration and the additive CaCl₂ had little effects on the reaction.Conclusion: The optimum conditions of enzymatic safflower oil hydrolysis should decide by reaction time.更多还原