【摘要】 目的:建立人c-myc转基因细胞。方法:通过成功构建c-myc逆转录病毒表达载体,并经脂质体介导转染包装细胞293T,收集产重组病毒的293T培养上清,运用NIH3T3细胞测定了病毒滴度,用适当浓度的病毒感染L929细胞,经用Zeocin选择性培养基筛选细胞。结果:得到稳定高表达c-myc基因的L929转基因细胞。结论:运用逆转录病毒转染法可得到高表达的转基因细胞。更多还原

【Abstract】 Objectives:To construct human c-myc transgene cell.Methods: Human c-myc cDNA was recombinated into retroviral vector plasmid pGEZ-Term.The recombinant of pGEZ-Term/c-myc and HIT45 and HIT60 were then transduced into the packaging cell line(293T) by means of lipofectin.Recombinant viruses were obtained.The retroviral titer was detected by infecting NIH3T3 cell with supernatants of 293T cell.The recombinant viruses infected L929 cell.By using the medium containing Zeocin for clone selection.Results: c-myc transgene cells were constructed.In addition,the supernatants of the L929 cells transduced with retroviral vector containing c-myc cDNA could significantly promote the proliferation of L929 cells.Conclusion:Transgene cells were obtained by retroviral transfer.更多还原