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氮离子束注入对燕麦M1-M2代幼苗耐盐性的影响
Effect of M1-M2 generation Oat Seedlings on Salt-tolerance after Implanted by N+ Ion Beams
【摘要】 采用能量为25keV的不同剂量(4×1016N+/cm2、6×1016N+/cm2、8×1016N+/cm2、12×1016N+/cm2)N+注入燕麦种胚,研究N+离子束注入后,盐胁迫对燕麦M1和M2代幼苗体内超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,脯氨酸含量、丙二醛(MDA)含量、可溶性糖及可溶性蛋白含量的影响。实验结果表明:对M1代,以6×1016N+/cm2剂量处理燕麦较合适,能提高燕麦受盐胁迫影响后幼苗SOD活性和可溶性糖含量。而对M2代,4×1016N+/cm2处理剂量能降低MDA含量,提高可溶性糖含量,这都有利于盐胁迫后燕麦幼苗的生长。
【Abstract】 Embryos of Oat seed were implanted with different dosage of N+ at the energy of 25keV.The dosage ranged from 4×1016 N+/cm2 to 12×1016 N+/cm2.The effects on superoxide dismutase(SOD),peroxidase(POD),catalase(CAT) activities of M1 and M2 generation of Oat seedlings were studied,and contents of malondiadehyde(MDA),prolin contents,soluble sugar and soluble protein contents were checked up under NaCl stress.The results showed that: As M1 generation,it suggested that optimum dosage(6×1016 N+/cm2) of N+ ion beam implantation could raise superoxide dismutase(SOD) activity and soluble sugar contents,and promote seedlings of Oat to grow.But M2 generation,optimum dosage of N+ ion beam implantation was 4×1016 N+/cm2 which could brought down malondiadehyde(MDA) contents and improved soluble sugar contents,it were beneficial to grow seedlings of oat after NaCl stress.
【Key words】 N~+ ion beams Implantation; oat; M1 and M2 generation; Physiological and Biochemical characters;
- 【文献出处】 生物技术 ,Biotechnology , 编辑部邮箱 ,2006年02期
- 【分类号】S512.6
- 【被引频次】8
- 【下载频次】88