【摘要】 目的:比较不同方法提取苛求芽孢杆菌基因组DNA的差异。方法:用经典CTAB提取法、改进CTAB法(溶菌酶处理结合CTAB提取法)、UniQ柱吸附提取法制备苛求芽孢杆菌基因组DNA,比较产物完整性和用于PCR扩增的有效性。结果:三种方法制备基因组DNA纯度接近,但改进CTAB法产率最高,UniQ法产率最低。经典CTAB法和UniQ法提取基因组DNA易降解。三种方法所得基因组DNA用于PCR扩增效率接近。结论:溶菌酶裂解结合CTAB提取更适合制备苛求芽孢杆菌基因组DNA。更多还原

【Abstract】 Objectives:To compare methods for the preparation of genomic DNA from Bacillus fastidious.Methods: Genomic DNA was extracted by classical CTAB method,modified CTAB method with the use of lysozyme and UniQ ion-exchange method,the quality and quantity of genomic DNA were compared.Results: The modified CTAB method showed the highest yield,while the UniQ ion-exchange method showed the lowest yield of genomic DNA.These three methods gave DNA preparation of comparable homogenecity and efficiency as the template for PCR amplification.Conclusion: The modified CTAB method was better for preparing genomic DNA from Bacillus fastidious.更多还原