【摘要】 目的:为了利用基因遗传转化改良小麦品质,采用聚合酶链式反应(PCR)技术。方法:从小麦品种东农7742基因组DNA中扩增并克隆了小麦高分子量谷蛋白12亚基基因(HMW-GS 12)。结果:序列分析结果表明,该基因全长1 980bp,其核苷酸顺序和推导的氨基酸顺序与已发表的序列相比,同源性分别为99.5%和99.7%。经过基因拼接,分别构建了胚乳特异性表达和组成型表达的高分子量谷蛋白12亚基基因的两个植物表达载体pDNPPBIHG和pUbPBIHG。更多还原

【Abstract】 Objectives:To improve wheat quality by gene genetic transformation.Methods:High molecular weight glutenin 12 subunit gene(HMW-GS 12) in wheat was amplified and cloned from wheat cultivar NE7742 genomic DNA by polymerase chain reaction.Results: Sequencing analysis showed the cloned fragment contained 1980 nucleotides and shared a homology of 99.5% and 99.7% respectively in DNA sequence and deduced amino acid sequence with the sequence published on genbank.By DNA processing,two plant expression vectors were constructed that one was endosperm-specific expression vector and one constitutive expression vector pDNPPBIHG and pUbPBIHG, respectively.更多还原