【摘要】 建立了一种快速、准确的高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定发酵液中L-精氨酸含量。采用Prevail C18色谱柱(C18 5μm,250×4.6 mm,Alltech),以5 mmol.L-1七氟丁酸(三氟乙酸调pH至1.0)和乙腈为流动相,采用梯度洗脱,总流速为1.0 mL/min。ELSD参数:漂移管温度为117.0℃,载气流速为3.2 L/min。L-精氨酸等氨基酸的回收率为96%~104%。能够快速、精确测定发酵样品中目的产物L-精氨酸与其它氨基酸含量。更多还原

【Abstract】 A HPLC-ELSD method was established for determination of L-arginine in fermentation broth.Prevail C18 column（C18 5 μm,250 mm×4.6 mm,Alltech）was used and heptafluorobutyric acid（pH 1.0 adjusted by trifluoroacetic acid） and acetonitrile were used as mobile phase with gradient elution,and total flow rate was 1.0 mL·min^-1.ELSD parameter: The temperature of drift tube was 117.0 ℃ and flow rate of carrier gas was 3.2 L·min^-1.The recovery of L-arginine and other amino acids were in the range of 96%～104%.By this method Larginine and other amino acids could be determined rapidly and effectively.更多还原