【摘要】 从微观体系考察了在双底物抑制下脂肪酶催化合成乳酸乙酯,叔丁醇是最佳溶剂,它可以很好地破坏乳酸分子由缔合而形成的分子簇。在所选的脂肪酶中,Novozym 435(南极假丝酵母脂肪酶B)的催化活性最好。综合产率和酶活两方面因素确定了最佳实验条件:酸醇摩尔比=1∶8、反应温度60℃、酸浓度0.3 mol/L、酶浓度45 g/mol、摇床转速200 r/min。更多还原

【Abstract】 Ethyl lactate was synthesized by lactic acid and ethanol in double substrates inhibition,and the best solvent was tert-butyl alcohol,which can break the force associated between lactic acid moleculars the view of microcosmic,and the best lipase is Novozym 435(Lipase B from Candida antarctica).The optimized conditions were studied and confirmed in detail according to yield and activity of enzyme:the molar ratio(acid∶alcohol) was 1∶8,the temperature was 60 ℃,the concentration of acid and lipase was 0.3 mol/L and 45 g/mol,respectively,the rotatation speed was 200 r/min.更多还原