【摘要】 以来自于巨大芽孢杆菌的细胞色素P450BM-3为研究对象,采用随机突变和饱和定点突变定向进化技术对P450BM-3进行改造,通过突变体催化靛蓝显色的特性采用活性琼脂平板分析和96微孔板相结合的高通量筛选成功获得了几个具有更高催化性能的突变体。更多还原

【Abstract】 Directed evolution is a new developed approach to protein modification engineering.Cytochrome P450BM-3 from B.Megaterium was evolved by random mutagenesis and Site-saturation mutagenesis.Some P450BM-3 mutants were obtained by high through-put screening of the combination with the active agar plates with some amount of substrate indole and 96-well microtile plates of catalytic activities according to colourimetric product formation of blue pigment indigo that the P450BM-3 mutants are able to produce.更多还原