【摘要】 对玉米Hz85(O2/O2)和Hz85(o2/o2)以及S7913(O2/O2)和S7913(o2/o2)两种不同核背景下的近等基因系(NIL),采用cDNA芯片杂交技术研究玉米o2突变基因及赖氨酸形成和胚乳发生相关基因在RNA水平上的表达差异.在两套NILs中检测到共同的表达差异克隆87个,对其序列分析得到26个TUGs(tentativeuniquegenes)、11个未命名蛋白和6个新序列.这些TUGs分别参与了生长发育、胁迫响应、物质运输、信号转导、电子传递链、细胞防御、代谢等细胞过程,以及作为细胞组分和贮存蛋白.基于O2/o2NILs间胚乳发育中基因表达的差异,讨论了o2籽粒中的不透明粉质胚乳形成的分子机制.更多还原

【Abstract】 The differences at transcription level of the maize o2 mutation and genes related with the lysine formation and endosperm development were examined by cDNA microarray in two pairs of maize near-isogenic lines (NILs), Hz85(O2/O2) vs Hz85(o2/o2) and S7913(O2/O2) vs S7913 (o2/o2), respectively. Eighty-seven clones detected in both pairs of NILs were sequenced. Bioinformatics analyses showed that these clones represent 26 TUGs (tentative unique genes), 11 unnamed proteins and 6 new sequences. The TUGs are genes involved in several cell processes such as development, stress response, transportation, signal transduction, electron transfer chain, apoptosis, cell defense, metabolism, cell components and storage proteins. The molecular mechanisms of the formation of a soft, starchy, opaque mature endosperm in the o2 kernels were discussed based on the O2/o2 NILs.更多还原