节点文献

玉米DEAD-box RNA解旋酶基因的克隆及分析

Cloning and Characterization of DEAD-box RNA Helicases Gene from Maize (Zea mays L.)

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 张珊珊陶勇生郑用琏张方东

【Author】 ZHANG Shan-Shan, TAO Yong-Sheng, ZHENG Yong-Lian, ZHANG Fang-Dong*(National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China)

【机构】 华中农业大学作物遗传改良国家重点实验室华中农业大学作物遗传改良国家重点实验室 武汉430070武汉430070

【摘要】 DEAD-box RNA解旋酶参与RNA转录、前体mRNA剪切、核糖体发生、核质运输、蛋白质翻译、RNA降解等重要的生命活动.根据本室在S-Mo17Rf3Rf3cDNA芯片研究中,检测到花粉发育后期RNA解旋酶上调表达的结果,应用RACE技术从S-Mo17Rf3Rf3花粉中克隆得到该RNA解旋酶基因全长cDNA,命名为ZmRH2并在GenBank注册登记(DQ327709).序列分析表明该cDNA全长1652bp,从第163bp开始到1386bp含有一个开放阅读框,编码407个氨基酸.其编码的蛋白质具有DEAD-boxRNA解旋酶特有的9个保守模体,与水稻、拟南芥和豌豆中的DEAD-boxRNA解旋酶的氨基酸序列存在着很高的同源性.RT-PCR分析表明,该基因在近等基因系S-Mo17Rf3Rf3和S-Mo17rf3rf3的叶、根、和雌穗中的表达没有差异,但在花丝和花粉中有明显差异.

【Abstract】 DEAD-box RNA helicases are involved in various aspects of RNA metabolism, including RNA transcription, premRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation and RNA decay. According to maize S-Mo17 Rf3Rf3 cDNA microarray data, an RNA helicases was found to be upregulated in the later stage of pollen development. Full-length cDNA of this RNA helicases, named ZmRH2 was cloned (GenBank accession number: DQ327709) in maize S-Mo17 Rf3Rf3 pollen using RACE. cDNA sequencing indicated: the full length of ZmRH2 cDNA is 1 652 bp, which contained an open reading frame encoding 407 amino acids from 163th to 1 386 th positions. The encoding amino acid sequence of ZmRH2 consisted of nine conserved motifs that characterize DEAD-box RNA helicases and showed highly homology with DEAD-box RNA helicases of Oryza sativa, Arabidopsis thaliana and Pisum sativum. RT-PCR showed that expression level of ZmRH2 showed little difference in the leaf, root and ear between one pair of maize NIL (Near Isogenitic lines) of S-Mo17 Rf3Rf3 and S-Mo17 rf3rf3 , and the expression of ZmRH2 differed significantly in silk and pollen between S-Mo17 Rf3Rf3 and S-Mo17 rf3rf3 .

【基金】 国家重点基础研究发展规划(973计划项目)资助(No.2001CB1088-4)~~
  • 【文献出处】 中国生物化学与分子生物学报 ,Chinese Journal of Biochemistry and Molecular Biology , 编辑部邮箱 ,2006年08期
  • 【分类号】S513
  • 【被引频次】28
  • 【下载频次】416
节点文献中: 

本文链接的文献网络图示:

本文的引文网络