【摘要】 为了进一步探讨SO2的毒理学作用机制,运用荧光实时定量RT-PCR和免疫组化技术研究SO2吸入对大鼠肝细胞中p53、bax和bcl-2三种细胞凋亡相关基因mRNA和蛋白表达的影响.结果显示,肝中p53和baxmRNA水平呈剂量依赖性增加,在SO2浓度为28·00和56·00mg/m3时显著增加(p53mRNA在28mg/m3为1·30倍,在56mg/m3为3·43倍;baxmRNA在28mg/m3为1·63倍,在56mg/m3为2·17倍);而bcl-2mRNA水平显著降低(在28mg/m3为0·63倍,在56mg/m3为0·45倍).免疫组化实验结果表明,吸入SO2后,大鼠肝中p53和bax蛋白表达水平呈剂量依赖性增加,而bcl-2蛋白表达水平呈剂量依赖性降低.结果表明,SO2可以改变凋亡相关基因的表达,诱导大鼠肝组织细胞凋亡,这可能与一些凋亡相关疾病的发生有关.更多还原

【Abstract】 The mRNA and protein levels of p53, bax, bcl-2 were analyzed in livers using a real-time reverse transcription-polymerase chain reaction （real-time RT-PCR） assay and immunohistochemistry method, respectively.Our results showed that mRNA levels of p53 and bax were increased in a dose-dependent manner and at the SO₂ concentrations of 28.00 and 56.00 mg/m³ their increases were significant, while mRNA levels of bcl-2 were decreased significantly in livers of rats exposed to SO₂.Dose-dependent increases of p53 and bax proteins in the livers were observed after SO₂ inhalation, while decrease of bcl-2 protein levels was obtained using immunohistochemistry method.These results lead to a conclusion that exposure SO₂ can change the expression of apoptosis-related genes, and it suggests that SO₂ can induce apoptosis in liver of rat and may be related with some apoptosis-related diseases.Elucidating the expression patterns of those factors after SO₂ inhalation might be critical to our understanding mechanisms of SO₂ toxicity and helpful for the therapeutic intervention.更多还原