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微生物发酵产辅酶Q₁₀的高速逆流色谱法分离纯化

Separation and Purification of Coenzyme Q₁₀ from Microbial Fermentation by High-speed Counter-Current Chromatography

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【Author】 CAO Xue-li1 XU Ya-tao1 ZHANG Guang-ming2 XIE Sheng-meng2 DONG Yin-mao1(1 Beijing Technology and Business University,School of Chemical and Environmental Engineering,Beijing Key Lab of Plant Resource Research and Development Beijing 100037,China)(2 Space Biology Lab,Chinese Academy of Space Technology Beijing 100081,China)

【机构】北京工商大学化学与环境工程学院北京市植物资源研究开发重点实验室；中国空间技术研究院空间生物实验室；北京工商大学化学与环境工程学院北京市植物资源研究开发重点实验室北京100037；北京100037；北京100081；

【摘要】首次将高速逆流色谱法应用于微生物发酵液提取物中辅酶Q10的分离纯化,建立了一套可用于其制备分离的逆流色谱溶剂体系正庚烷-乙腈-二氯甲烷(12∶7∶3.5,v/v/v)。500mg发酵液粗提物经一步制备分离,可得到绝对纯度在98%以上的辅酶Q10130mg。比较表明,该方法较传统的硅胶柱层析和结晶相结合的纯化方法在产物纯度、回收率及产率等方面都有一定的优势。更多还原

【Abstract】 High-speed counter-current chromatography （HSCCC） is applied for the first time in purification of coenzyme Q₁₀(CoQ₁₀) from a fermentation broth extract. A non-aqueous two-phase solvent system composed of heptane-acetonitrile-dichloromethane （12∶7∶3.5,v/v/v） is selected by analytical HSCCC and used for preparative purification of CoQ₁₀ from 500 mg of the crude extract. The separation yielded 130 mg of CoQ₁₀ at high purity of over 98%. The overall results of the present studies show advantages of HSCCC in the purity,recovery and yield of CoQ₁₀ over the silica gel chromatography with subsequent crystallization.更多还原