【摘要】 PS1基因突变与早发家族性老年痴呆有密切联系。构建pEGFP-C1-PS1以及pEGFP-N2-PS1融合基因表达载体,于HEK293和CHO细胞系中表达PS1/GFP融合蛋白,以GFP绿色荧光作为PS1的亚细胞定位信号,通过SPOTII以及CONFOCAL显微镜进行观察,初步获得PS1全长蛋白在细胞中定位的部分信息,即PS1定位于细胞核膜,细胞质内有不均匀的分布,少量存在于细胞-细胞接触处的细胞膜上。更多还原

【Abstract】 Mutations in presenilin1 (PS1) gene are genetically linked to the early onset of familial Alzheimer’s disease (EOFAD). The fusion gene GFP-PS1 (recombinant plasmid pEGFP-C1-PS1) and PS1-GFP (recombinant plasmid pEGFP-N2-PS1) were constructed to study the cellular distribution of PS1 holoprotein. Recombinant plasmids were transiently transfected into HEK293 cell and CHO cell, and watched with SPOTⅡ(Olympus,BH2) and CONFOCAL microscope (Olympus, FV300) under 488nm. The PS1 locates on nuclear envelope, fewer on cellular membrane, and inhomogeneous distribution in cytosol.更多还原