【摘要】 研究了荧光素与牛血清白蛋白(BSA)相互作用的荧光光谱及其最佳测试条件和影响因素。在pH2.2,荧光素-牛血清白蛋白复合物的荧光强度最大,其激发波长(Ex)为467nm,发射波长(Em)为515nm。在BSA1.8~500mg/L范围内,该复合物荧光强度与BSA浓度成线性关系,F=25.776C+2.8082,r=0.9999。该法简单、快速、灵敏、稳定、成本低。更多还原

【Abstract】 A fluorescence method is found for determination of bovine serum albumin(BSA).The method is based on the interaction of fluorescein with BSA to form a complex in Tris-HCl buffer of pH 2.2. The complex’exitation wavelength is 467 nm,the emission wavelength is 515 nm. The linear measurement rang is between 1.8~500 mg/L for BSA, F=26.776C+2.8082,r=0.9999.This method is sensitive,steady,and low cost for determination of BSA.更多还原