【摘要】 为了克服现有被动式生物芯片的不足,我们从抗原抗体反应的基本原理出发,建立了固相表面的抗原抗体反应模型,并根据模型设计了一种新型的主动式蛋白芯片系统,该系统中引入负压发生装置及控制装置,可对硝酸纤维素(NC)膜上发生的抗原抗体反应进行控制。并对该系统进行了仿真,结果表明它具有快速、稳定、鲁棒性好等优点,能满足实际要求。该系统将有助于提高抗原抗体反应的效率,改善芯片检测结果的重复性和准确性。更多还原

【Abstract】 To overcome the present limitations of passive biochip,based on the basic principle of antigen-antibody reaction,we develop an antigen-antibody reaction model of solid-phase surface and design a novel active biochip system according to this model,which introduces the negative pressure and controlling devices to control the immunoreactions on the nitrocellulose(NC) membrane.From the computer simulation results,this is a rapid,stable,robust and practicable system,which can be used to increase the efficiency of immunoreactions and improve the reproducibility and accuracy of biochip analysis.更多还原