【摘要】 电压敏感染料的膜电位光学标测是一项集分子光子学、生理科学、计算机科学等诸多学科为一体的高科技技术手段。与传统的微电极、膜片钳技术相比,它不仅可以多位点、同步测量膜电位的变化,而且可以标测细胞群电活动的传导特性,为研究较小细胞和纤细的神经突起、心律失常等生理活动提供了可能。由于它可以避免外部电磁场的干扰,因此已成为研究心脏电除颤等机理较为理想的手段。本文重点介绍了电压敏感染料膜电位光学标测技术的工作原理、系统构架以及目前的主要应用及其未来的发展方向。更多还原

【Abstract】 Optical mapping of the membrane potential with voltage-sensitive dyes is an advanced approach that involves in many theories such as molecular photonics,physiology and computer science.Compared with the conventional techniques of membrane patch-clamp and microelectrode,the optical mapping system can measure not only the changes of a single membrane potential from multiple sites simultaneously but also the conduction properties of the cell populations.So this technique provides an important method for studying the electrophysiology of the small cell,the tiny neurite and the cardiac arrhythmia,etc.Because this technique can also avoid the electromagnetic interferences,it uniquely provides an ideal means for studying the mechanisms such as cardiac defibrillation.In this paper the principles and the system structure of the optical mapping are introduced,its applications and future developments are also presented at the same time.更多还原