【摘要】 研究了旋转生物反应器的力学环境及其对细胞生长的影响。理论建模和微分方程求解反应器内重力和细胞所受剪应力的大小,扫描电镜评价细胞生长密度、生长速度和形貌。结果表明,自制的旋转生物反应器在理论上能提供微重力(K<8.38×10-2)和低剪应力(τ<1.62 dyn/cm2)环境,扫描电镜结果显示在反应器内培养的细胞生长速度、数量和形态明显优于24孔板静态培养。旋转生物反应器的力学环境有利于细胞保持良好形态和快速扩增,是一种明显优于静态培养的新方法和新技术。更多还原

【Abstract】 In this paper is presented an analysis of the mechanical effect of horizontal rotating bioreactor on cell culture.Getting the microgravity of the bioreactor and the shear stress on canine mesenchymal stem cells（cMSCs） with theoretic calculating model and differential equations,we have validated the density,growth rate and modality of cultured cell by scanning electron microscopy.The horizontal rotating bioreactor which we developed could create the mechanic environment of microgravity(K<8.38×10^-2)and low shear stress（τ<1.62 dyn/cm²） in theory.The results of scanning electron microscopy indicated that the cells’ growth-speed,quantity and modality in bioreactor were better than those of cells cultured in static 24-well plate.The mechanical environment of the rotating bioreactor is propitious for keeping better modality and more rapid proliferation of cMSCs.The rotating bioreactor is a novel approach and technique;it is superior to static culture.更多还原