【摘要】 细胞培养是一种体外研究活组织和活细胞的常用方法之一,而体外培养细胞的活性评价在体外细胞培养的研究中非常重要。现阶段主要使用医学和生物学的手段对细胞进行活性检测,不仅成本高,处理麻烦,且每次检测都会损失大量的细胞。本文提出了一种基于图像处理与分析方法的评价方法,在不破坏细胞的情况下获取活体细胞图像,通过对图像进行预处理和阈值化分割提取出生长晕,对其面积进行统计分析评价细胞的活性。对骨髓间充质干细胞的试验结果表明本文评价方法是准确有效的,能够大大降低细胞活性评价的成本,克服了传统评价方法破坏细胞的缺点。更多还原

【Abstract】 Cell culture is one of the usual methods for studying living cell and tissue,the method presented in this paper is based on image processing and analyzing technology for activity estimation of mesenchymal stem cells(MSCs).The existing activity estimation methods are costly,complex and invasive.In this method,thresholding is used to preprocess image and to separate out the growth hallow. Then the area is calculated by counting the pixels of the growth hallow.The changes of the activity estimated by this method are similar to those by corresponding cellular experiments.Compared with the existing methods in biology,medicine or medical cellular science,this method is easier,faster,cost-effective and noninvasive.The proposed method has been proved to be efficient by primary experiments of MSCs.更多还原