【摘要】 评述了当前国际上嗅觉和味觉组织、细胞电生理及其芯片技术的研究状况。介绍了细胞胞内记录技术在嗅觉和味觉的电生理研究中应用,分析该技术在实现组织-细胞以及胞间信号传导过程的实时动态检测中面临的问题。在此基础上,进一步介绍细胞阵列和单细胞芯片技术的特点及目前的发展,指出了芯片技术应用于嗅觉和味觉组织的发展前景。最后结合我们的工作,提出嗅觉和味觉细胞芯片的研究和发展方向。更多还原

【Abstract】 This paper introduces the new research achievement and progress of electro-physiology in olfaction and gustation.Classical implements such as patch-clamp or glass pipette are not appropriate in the dynamic detection of cellular signal transportation.In view of this,we have analyzed the feasibilities and challenges of olfactory or gustatory cell-based biosensors such as field effect transistor(FET) and light addressable potentiometric sensor(LAPS).Finally we present the research work carried our in out lab and a future prospective on the development in this field.更多还原