【摘要】 目的：研究原癌基因c-met及其相关基因在大鼠胰腺发育及细胞功能完善过程中的表达。方法：采用高密度寡核苷酸芯片(Affymetrix芯片)对孕12．5(E12．5)、E15．5和E18．5、新生、成年胰腺进行基因转录水平分析,并用RT-PCR验证基因在大鼠胰腺不同发育时期的表达。结果：c-met基因在E15．5、E18．5较成年特异高表达。芯片中c-met转录调控基因和信号传导通路相关基因的表达趋势与c-met高度相似。RT-PCR(所用引物设计区域与芯片相同)验证,c-met表达趋势与芯片结果相符；与芯片c-met探针所用引物不同RT-PCR,结果却在各发育阶段呈现与芯片不同的表达趋势。结论：提示c-met可能在胰腺发育细胞功能完善的关键阶段起调控作用,参与胚胎胰腺发育中晚期细胞功能完善的信号传导过程。并且c-met在胰腺发育中发挥作用有可能存在不同转录本。更多还原

【Abstract】 Objective:To investigate the function of c-met and its related genes in the different development stages of rat pancreas. Methods:Gene Chip Expression Set230A was used to detect the signal value of different devdopment stages of rat pancreas(E12.5,E15.5, E18.5,newborn,adult).RT-PCR was used to verify the results and to detect different transcription of c-met.Results:C-met was ex- pressed highly at rat embryonic day 15.5,18.5.The c-met expression trendency of Gene Chips was consistent with result of RT-PCR,but dif- ferent primer of RT-PCR has another result.Conclusion:C-met proto-oncogene may plays a role in the function maturation stages of rat pan- creatic development.There may be different transcriptions of c-met in the progression of rat pancreatic maturation.更多还原