【摘要】 目的:测定北五味子及其不同炮制品中五味子甲素、五味子乙素的含量。方法:采用高效液相色谱法,Spherisorb 10ODS1(4.6mm×250mm,10μm),以甲醇-水(70:30)为流动相,检测波长为254nm。线性范围0.1~0.5μg,相关系数为0.995.平均回收率为100.3%和100.4%,RSD=0.87%和2.85%(n=5)。结果:五味子甲素和乙素在生品、醋炙、炒制、酒炙、蒸炙、蜜炙品中的平均含量分别为0.13%,0.064%,0.052%,0.064%,0.11%,0.13%和0.21%,0.298%,0.238%,0.302%,0.216%,0.214%。结论:方法准确,快速,重现性好,可用于五味子各种炮制品的质量评价。更多还原

【Abstract】 To compare the content of deoxyschizandrin andγ-schizandrin in the raw medical material and the different processd products of Fructus Schisandrae Chinensis(FSC).Methods: The content of deoxyschizandrin andγ-schizandrinwere was determined by HPLC(=high performance liquid chromatography),with Spherisorb 10 ODS1(4.6mm×250mm,10μm),the mobile phase of a mixture of methanol-water(70:30),and detecting wave length of 254nm.The standard curve was linear in the range of 0.11～0.55μg,with correlation coefficient 0.995.The average recovery of FSC was 100.3% and 100.4%,with RSD=0.87% and 2.85%.Results: The mean content of deoxyschizandrin was 0.13%,0.064%,0.052%,0.064%,0.11%,0.13% andγ-schizandrin,0.21%,0.298%,0.238%,0.302%,0.216%,0.214%,in the different processed products of FSC.Conclusion: The method is accurate,quick and good reappearance,which can be used for the quality evaluation in the different processed products of FSC.更多还原