【摘要】 目的:观察N-乙酰转移酶NAT10蛋白在软组织肉瘤中的表达及与类型、分级的关系。方法:通过原核表达NAT10蛋白免疫制备特异性多克隆抗体,并经免疫印迹鉴定;以组织芯片-免疫组化检测166例软组织肉瘤和28例良性肿瘤及瘤样病变中NAT10蛋白的表达。结果:制备多克隆抗体经Western印迹鉴定与NAT10具有特异结合性。免疫组化显示166例软组织肉瘤中NAT10蛋白阳性95例,阳性率为57%(95/166),28例良性肿瘤及瘤样病变中4例阳性14%(4/28),两者间有显著性差异(P<0.05)。NAT10表达的主要分布为:滑膜肉瘤76%(13/17)、恶性纤维组织细胞瘤75%(15/20)、原始神经外胚叶瘤(PNET)70%(16/23)、横纹肌肉瘤70%(7/10)、恶性外周神经鞘膜瘤50%(11/22)、隆突性皮肤纤维肉瘤50%(7/14)、平滑肌肉瘤43%(6/14)、脂肪肉瘤42%(8/19)、黏液性纤维肉瘤38%(6/16)。统计比较显示:滑膜肉瘤与黏液性纤维肉瘤和脂肪肉瘤,以及恶性纤维组织细胞瘤与黏液性纤维肉瘤之间NAT10表达具有显著性差异(P<0.05);而其它各组间无明显差异(P>0.05)。同时,NAT10蛋白强阳性表达(≥++)多存在于滑膜肉瘤(53%,9/17)、横纹肌肉瘤(40%,4/10)及恶性纤维组织细胞瘤(40%,8/20)。在FNCLCC分级中,19例I级肉瘤中NAT10阳性表达率为42%(8/19),44例II级肉瘤为43%(19/44),70例III级肉瘤为73%(51/70)。III级NAT10阳性率显著高于II级组和I级组(均为P<0.05)。结论:研究表明N-乙酰转移酶NAT10表达于多种人软组织肉瘤,尤其在高度恶性肉瘤,因此有可能为软组织肉瘤的分级及预后因子。更多还原

【Abstract】 Objective: To evaluate the expression of N-acetyltransferase NAT10 protein in human tumors of soft tissue and its significance.Methods: Anti-NAT10 polyclonal antibody was generated by immunization of E.coli expressing NAT10 protein.The expression of NAT10 in 166 cases of soft-tissue sarcoma(STS) and 28 cases of benign tumors or tumor-like lesions of soft tissue arranged in tissue chip was analyzed by immunohistochemistry.Results: Polyclonal antibody obtained from immunized rabbit was verified its specific reactivity with native NAT10 protein by Western blot. The immunohistochemical staining of NAT10 showed that about 57%(95/166) of STS were positive and only 14%(4/28) for benign tumors or tumor-like lesions,there was significant difference between STS and benign lesions.The positive distribution in NAT 10 expression was mainly synovial sarcoma 76%(13/17),malignant fibrous histiocytoma 75%(15/20),rhabdomyosarcoma 70%(7/10),primitive neuroectodermal tumor(PNET) 70%(16/23),dermatofibrosarcoma protuberans 50%(7/14),malignant peripheral nerve sheath tumor 50%(11/22),leiomyosarcoma 43%(6/14),liposarcoma 42%(8/19) and myxofibrosarcoma 38%(6/16).The statistical analysis showed that NAT10 expression of synovial sarcoma was different from those of myxofibrosarcoma or liposarcoma(P<0.05),and malignant fibrous histiocytoma from myxofibrosarcoma(P<0.05),but no differences in other groups(P>0.05).Meanwhile,strong positive staining of NAT10(≥++) was much frequently in synovial sarcoma(53%,9/17),rhabdomyosarcoma 40%(4/10) or malignant fibrous histiocytoma 40%(8/20).In FNCLCC grading,the positive expression rates of NAT10 were 42%(8/19) in 19 cases of grade1 sarcoma,43%(19/44) in 44 cases of grade2,and 73%(51/70) in 70 of grade3,which was significantly higher than those in grade 1 or 2(P<0.05).Conclusions: NAT10 protein could be detected in many kinds of STS,especially in high histological grading,and thus it could be a potential factor for grading or prognosis of STS.更多还原