【摘要】 阿维拉霉素产生菌SV微波诱变效应的研究发现:菌株SV对微波敏感(微波处理60s,SV菌株存活率低于10%),菌落形态变化大。微波诱变处理最佳作用方式为培养皿不加盖、快速冰上冷却,最佳处理时间为50s(其正突变率25.3%)。通过微波诱变处理、阿维拉霉素推理筛选,最终获得一株稳定性良好,阿维拉霉素产量达到21.5mg/L,较出发菌株提高119.4%的突变株SV-15。更多还原

【Abstract】 To investigate Mutagenic effects of Avilamycin-producing Strain induced by microwave irradiation.The experimental results indicated that the capacity of SV strain to microwave irradiation was sensitive(its survival rate was under 10% for 60s radiation).Not sealing the culture dish and fast cooling it was the optimal irradiation mode of microwave,and 50 seconds was the optimal irradiation time of microwave(positive mutation rate,25.3%).To obtain high-yield avilamycin-producing strain,50s microwave irradiation and screening of avilamycin-rational mutant is used for the study on screening mutation.SV-15 mutants were obtained,and the mutant could produce 21.5mg/L avilamycin,which was 2.194 times as much as the potency of original strain by flake fermentation.更多还原