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不同形态的磷源对球形棕囊藻生长及碱性磷酸酶的影响

Effects of Different Phosphorus on the Growth and Alkaline Phospohatase Activity in Phaeocystis Globosa

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【作者】 王艳唐海溶

【Author】 WANG Yan1,TANG Hai-rong2(1.Department of Environmental Engineering,College of Science & Engineering,Jinan University,Guangzhou 510632,China;2.Institute of Hydrobiology,Jinan University,Guangzhou 510632,China)

【机构】 暨南大学理工学院暨南大学水生生物研究所 广州510632广州510632

【摘要】 研究了不同浓度和形态的无机磷(DIP)和有机磷(DOP)对球形棕囊藻细胞生长、培养基中磷浓度的变化以及碱性磷酸酶活性的变化的影响。在缺磷和大分子DOP(卵磷脂)作为磷源的情况下,碱性磷酸酶活性迅速提高,在第9天达到了最大值,其活性分别为19.11U和18.28U,显然在缺磷胁迫下和大分子DOP的利用过程中碱性磷酸酶起着重要作用。DIP(磷酸二氢钾)和小分子DOP(β-甘油磷酸钠)都容易直接被球形棕囊藻吸收利用,碱性磷酸酶活性变化不明显。

【Abstract】 The growth of Phaeocystis globosa,total phosphorus in water,alkaline phosphatase activity in P.globosa cells were measured under different phosphorus conditions.When phosphorus deficiency and large molecular DOP(Lecithin)used in this experiment,alkaline phosphatase activity in P.globosa cells increased to 19.11U and 18.28U respectively,and both of them peaked at day 9.Results showed alkaline phosphatase played an important role in phosphorus deficiency stress and the utilization of large molecular of DOP.DIP(KH2PO4)and small molecular DOP(Natrium-β-glycerophosphat)can be easily utilized by P.globosa,whereas alkaline phosphatase activity did not changed significantly.

【基金】 国家自然科学基金30200030,30470283,30370126;国家科技部“973”赤潮项目2001CB409701;教育部留学回国人员科研启动基金[2005]383
  • 【分类号】X173
  • 【被引频次】44
  • 【下载频次】522
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