【摘要】 目的:观察肥大细胞分泌颗粒内特异性酶的分布及其超微结构,并探讨肥大细胞的功能。方法:用双重免疫胶体金标记类胰蛋白酶抗体(AA5)及类糜蛋白酶抗体(CC4),在透射电子显微镜下观察肥大细胞内分泌颗粒的超微结构。结果:在肥大细胞内分泌颗粒中富集类胰蛋白酶和(或)类糜蛋白酶,多数酶蛋白呈细小球形结构;在细胞膜内侧分泌颗粒融合形成分泌通道,细胞的外侧可见肥大细胞脱出的颗粒;细胞内的另侧分泌颗粒呈球形小泡。结论:肥大细胞内酶蛋白的富集可为肥大细胞参与Ⅰ型变态反应提供物质基础。更多还原

【Abstract】 Objective: To study mast cell function and observe heterozyme distribution and its ultrastructure in secretory granule of mast cell.Methods: Using doubling immuno-colloidal gold labeling of anti-tryptase(AA5)and anti-chymase(CC4),the ultrastructure of secretory granule in mast cell was observed by transmission electron microscope.Results: Secretory granule in mast cell included rich tryptase and(or)chymase.Many apoenzymes showed exiguous globular structure.Secretory granules fused to form secretory channel in cell membrane.Degranulation of mast cell could be shown near outer cell membrane.Another lateral secretory granules displayed sphere vesicle in cell.Conclusion: Apoenzyme enriching in mast cell can offer material basis for mast cell taking part in allergic reaction of Ⅰ type.更多还原